The influences of red wine in phenotypes of human cancer cells – PMC

German and Walzem, 2000; Ruf, 2003)nHighlight:t cell growth of different human cancer lines. These studies demonstrate that intaking suitable amounts of red wines may play a role in preventing cancer development.nHighlight:cancer cell growth and colony formation, while diluted ethanol at same concentration boosts cell growth.nHighlight:the efficiency repressing colony formation on breast cancer cells is more significant than those on esophageal carcinoma cells.nHighlight:Nucleolar hypertrophy is a consistent cytological feature of cancer cellsnHighlight:The red wines also cause death of the grew up cancer cells and inhibit Pol III gene transcription.nHighlight:In present study, we investigated the roles of red wine in human cancer cell growth, colony formation and RNA Pol III gene transcription.nHighlight:our results further demonstrate that the red wines more dramatically decrease the rates of cell growth and colony formation than liquor spiritsnHighlight:three red wines also markedly decrease the rate of cell growth of TSCMLnHighlight:The repression of the red wines is in a dose-dependent manner (from 6.25 mM to 100 mM). At the concentration of 25 mM, red wines #1 and #3 completely inhibits the cell growth of MCF-7 line, whereas ethanol at same dose increases MCF-7 cell growthnHighlight:diluted ethanol promotes cell proliferation at low concentrationnHighlight:inhibiting Pol III gene transcription is able to repress cell transformationnHighlight: red wine may contain some bioactive components and function potential to repress cancer development.nHighlight:three red wines dramatically reduce the rate of KYSE-510 cell growth from 6.25 mM to 100 mM (Fig. 2). This cell line to the mature wines (#1 and #3) is much more sensitive than those to the young wine (#2)nHighlight:Upregulation of Pol III genes is tightly linked to cell proliferation, cell transformation, as well as tumor formationnHighlight: (Yi et al, 2018) on multiple human cancer lines.nHighlight: red wines are divided into two types: mature wines (brick red) and young wines (intense violet).nHighlight:red wines at same ethanol concentration dramatically decrease the rates of cell growth.nHighlight:we randomly purchased three commercial red wines, two mature wines and one young wine, from a supermarket.nHighlight:This indicates that the red wine indeed plays some extent roles in inhibiting human cancer cell growthnHighlight:inhibition of cell growth by red wines is in a dose-dependent manner.nHighlight:effect of the red wines on human breast cancer cells (MCF-7 line) is much stronger than those of human esophageal carcinoma cells (KYSE-510 line). But, the capacity of the red wines killing cancer cells is less than their inhibition role in cancer cell growth.nHighlight:Red wine phenolics contain two major groups: flavonoids and non-flavonoids.nHighlight:human cancer linesnHighlight: red wines significantly repress cell proliferation of different human cancer lines from low dose to high dosenHighlight:the inhibition role of mature wine (#1 and #3) is much stronger than those of young wine (#2)nHighlight: main flavonoid compounds presented in red wine include several classes, such as flavanols [(epi) catechin], flavonols (e.g., myricetin andquercetin) and anthocyanins (e.g., malvidin-3-glucoside), while non-flavonoid compounds presented in red wine comprise phenolic acids, phenols and stilbenes. nHighlight:induction of Pol III genes by diluted ethanol is able to be inhibited by bioactive components in red wines.nSticky notes:Key!!nHighlight:mature wines (#1 and #3) are able to cause death of human breast cancer cells, as well as esophageal carcinoma cells.nHighlight:Diluted ethanol (200 proof) has widely been using to explore molecular mechanisms of alcohol-caused human health issues, including tumor development.nSticky notes:200 proof!!!nHighlight:diluted ethanol-feeding mouse promoted liver tumor developmentnHighlight:repressing cancer cell growth by the red wines are in a dose-dependent mannernHighlight:red wine also inhibit colony formation of human breast cancer and esophageal carcinoma cellsnHighlight:Target sites for alcohol-related carcinogenesis include the breast, esophagus, liver and multiple additional organsnHighlight:While a previous study indicated that the workers, who tested liquor and sprits to check its quality during the course of production, were not found hepatic fibrosis, cirrhosis and HCC in this special crowd, compared to those of other beverage consumptionsnSticky notes:Key pointsnHighlight:diluted ethanol increases Brf1 expression to enhance Pol III gene transcription, resulting in alteration of the cellular phenotypesnHighlight:all three red wines decrease the rates of cell proliferation, compared to diluted ethanol treatment.nHighlight:effects of repression on different human cancer lines is in a dose-dependent mannernHighlight:repress colony formationnHighlight:red wines also repress colony formation of human breast cancer and esophageal carcinoma cells and inhibit Pol III gene transcription.nHighlight:persons intake wines, but not ethanol.nHighlight:The results indicate that no matter which red wines, all of them significantly decrease the cell growth rates of human lung carcinoma and colon cancer lines nHighlight:red wines also dramatically inhibit RNA Pol III gene transcription, compared to diluted ethanol.nHighlight:moderate daily consumption of red wine was as a contributing factor to the observed lower incidence of coronary heart diseasenHighlight:The effects of mature wine (brick red) on these cancer cell phenotypes are much stronger than young wine (intense violet)nHighlight:mature wine (#1) dramatically decreased both cell growth of human breast (Fig. 3nLeft) and esophageal (Fig. 3nRight) cancers. Both red wines (#2 and #3) also markedly reduced the rates of cell growth of the two human cancer cell lines.nHighlight:red wines markedly decrease the rates of colony formation of the human breast cancer cellsnHighlight:wine is not equal to ethanolnHighlight:the effects of mature wine (brick red) on cancer cell phenotypes are much stronger than young wine (intense violet)nHighlight: Epidemiological studies have indicated a positive association between red wine ingestion and human health.nHighlight:our studies reveal that the red wines, particularly mature wines (#1 and #3), dramatically decrease the rates of humannHighlight:These studies imply that wines may play a role in inhibiting cancer development.nHighlight:white wine) have the potential inhibiting human cancer growthnHighlight:these new findings suggest that red wines may containn]]>